Off the top of my head:

• AsapSCIENCE (Both he/him) - A nice science channel run by a gay couple
• Jaiden Animations (she/her) - A really funny storytime animation channel. Jaiden is ace.
• Powered By Rainbows (he/him) - This is a queer education channel, run by Matt Haslam and often featuring his husband.
• Squaishey Quack (they/them) - A classic gaming channel, focusing mainly on Minecraft and Pikmin. Squaishey is bi, non-binary, and married to Stampy.
• EmKay (various) - This Reddit channel features several narrators. Lexi (she/her) is trans and lesbian, and Robin (he/him) is bi.
• Vivziepop (she/her) - The channel behind Hazbin Hotel and Helluva Boss. Vivienne Medrano is bi.
• Hank Green (he/him) - Hank Green runs what I would describe as a misc channel. He posts commentary, gaming, and vlogs. Hank is bi.
• Thomas Sanders (he/him) - Thomas is one of my favourite personalities. He started by making Vines, and then moved over to YouTube and TikTok. He is also active on Instagram and Tumblr. Thomas is gay.
• Jammidodger (he/him) - Jamie does mainly Reddit and reaction content, as well as LGBTQ+ focused stuff. He's also really wholesome. Jamie is a bi trans man.
• One Topic At A Time (he/him) - A very wholesome Reddit and Gaming channel that ever queer person I know likes. James (OT) is not queer himself, but he is a true ally and uploads almost exclusively queer content (apart from his gaming stuff).

I also have a channel (RandomWolf) and I am non-binary and abrosexual. However, it is an archive channel and, as such, it is not even slightly LGBT-focused. Maybe I'll do a pride video at some point, idk.