this post was submitted on 22 Mar 2025
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That allows visualization of PCRed product, which is a far cry from whole genome sequencing. You can visualize a target at a time with no sequence info. Using it for sequences would be extremely tedious and require a lot of runs.
Yup, I used to do PCR genotyping. 50 samples running the same setup would take me a whole 12-hour day at my lower end lab.
I can't imagine having to do 50 different ones with 50 different templates and having to adjust each one. ๐ And that's for 50 sequences. How many are there in a human?
About 25,000 coding sequences and a lot more non-coding.