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submitted 1 year ago* (last edited 1 year ago) by fossilesque@mander.xyz to c/houseplants@mander.xyz

Ignore the chaos in the background. ๐Ÿ˜… My dog came through and knocked everything over with the wiggles.

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[-] HeavyMitchel@lemmy.ml 7 points 1 year ago

Nice ! Are you certain the cat has nothing to do with the chaos ? They're well versed in the art of framing the dog.

[-] fossilesque@mander.xyz 4 points 1 year ago

Haha, he's a bit more like a piece of furniture at 15, but I wouldn't put it past him. ๐Ÿ˜…

[-] vicfic@iusearchlinux.fyi 2 points 1 year ago

Which camera did you use? Like, ik its not relevant but the pic is too good.

[-] fossilesque@mander.xyz 3 points 1 year ago

A Pixel 6! I always shell out for a good camera phone. It's the only thing I really care about when looking for a phone. :) In another life, I was almost a photographer.

Hi! Love the plant. What's a tissue culture start?

[-] fossilesque@mander.xyz 2 points 1 year ago
[-] Sal@mander.xyz 1 points 1 year ago* (last edited 1 year ago)

๐Ÿ˜ฑ Cool! This smells like the start of a new hobby....

[-] Doxatek@mander.xyz 2 points 1 year ago

Haha good luck! I do tissue culture for a living. You definitely can do it at home but you have a lot of work cut out for you

[-] Sal@mander.xyz 1 points 1 year ago

Haha, thanks! I have a few years of experience with home mycology (agar work/sterile technique) and I also keep lots of houseplants, so I'm thinking some of these skills might transfer when I do get into it. A reason why I'm interested in this is because some of the work I've read about fungi-root interactions was performed on fungi grown on root tissue cultures - which makes sense - but I don't really know how one would practically achieve that. So I'm very curious about it...

[-] Doxatek@mander.xyz 1 points 1 year ago

Gotcha. While never having cultured fungus (on purpose) plants definitely pose unique challenges. Such as hormone application. I also think, though I don't have extensive knowledge and fungal media composition, that the media for plants can need quite a lot more sugar which makes bacteria and fungi even more a problem.

Really cool you've already done it at home though. Did you do pressure cooker method

[-] Sal@mander.xyz 2 points 1 year ago

Such as hormone application.

Oh, I haven't worked with hormones before. That would be interesting.

Did you do pressure cooker method

Yeah, my pressure cookers are basically my autoclaves. And I use a still-air box (30L transparent plastic tub with holes) for pouring plates and transfers.

[-] Doxatek@mander.xyz 2 points 1 year ago

Oh yeah the hormones are cool you can make them do some weird stuff haha

[-] karmiclychee@sh.itjust.works 1 points 1 year ago

That's intense

[-] Botrychium@mander.xyz 1 points 1 year ago

nice one! did you do the tissue culture yourself?

[-] fossilesque@mander.xyz 2 points 1 year ago

Not me. :) An acquaintance got a few cultures and I pitched in for one of the starters.

this post was submitted on 09 Jul 2023
114 points (98.3% liked)

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