“It is possible to commit no mistakes and still lose. That is not a weakness... that is life.”

Bashir: "They broke seven of your transverse ribs and fractured your clavicle."

Garak: "Ah, but I got off several cutting remarks which, no doubt, did serious damage to their egos."

Two, are they're not surprisingly both from Garak:

Garak: The truth is usually just an excuse for lack of imagination.

Odo: You'd shoot a man in the back?

Garak: Well, it's the safest way, isn't it?

The scene where Quark compares the federation to root beer with Garak.

Q: "I want you to try something for me. Take a sip of this. It's called root beer. What do you think?"

G: "Oh! It's vile!"

Q: "I know. It's so bubbly, and cloying, and happy."

G: "Like the Federation..."

Q: "But you know what's really frightening? If you drink enough of it, you begin to like it. Like the Federation..."

G: "Insidious!"

Picard - ‘You can test that assumption at your convenience’ most political way to say f’ around and find out.

With the first link, the chain is forged. The first speech censured, the first thought forbidden, the first freedom denied, chains us all irrevocably.

Starfleet was founded to seek out new life – well, there it sits! ...waiting.

"I regret that we meet in this way. You and I are of a kind. In a different reality, I could have called you friend." -- The Romulan commander in Balance of Terror

"In every revolution, there's one man with a vision." -- Kirk in Mirror, Mirror

Lying is a skill like any other, and if you want to maintain a level of excellence you have to practice constantly.

"Darmok and Jalad at Tanagra! Temba, his arms wide. Shaka, when the walls fell."

Those lines for some reason resonate with me. That episode highlighted a divide I didn't know existed between myself and others.

I can't be any more clear about what I'm saying to you. Please understand so we can work on this problem together!

Ode to Spot is pretty classic.

"... and no one ever offered him a command. He learned to play it safe, and he never, ever, got noticed by anyone"

This line has been the basis of several career changing events for me, and overall it's worked out pretty well

One I quote often:

"What do I do?"

"FIND HIM AND KILL HIM"

There’s coffee in that nebula!

This night would be better spent preparing for the next life instead of rehashing the mistakes of this one.

-Worf

"Well double dumbass on you" - James Tiberius Kirk

"I am... fully functional."

Prune juice, a worriors drink!

Inspector Kashyk: "You created false readings!"

Janeway: "Oh, wasn't that the theme for the evening?"

From Counterpoint, my favorite episode

Shut up already, Morn.

• everyone

Sisko:

Do you know what the trouble is? The trouble is Earth-on Earth there is no poverty, no crime, no war. You look out the window of Starfleet Headquarters and you see paradise. It's easy to be a saint in paradise, but the Maquis do not live in paradise. Out there in the demilitarized zone all the problems haven't been solved yet. Out there, there are no saints, just people-angry, scared, determined people who are going to do whatever it takes to survive, whether it meets with Federation approval or not.

And one from Quark:

Let me tell you something about Hew-mons, Nephew. They're a wonderful, friendly people, as long as their bellies are full and their holosuites are working. But take away their creature comforts, deprive them of food, sleep, sonic showers, put their lives in jeopardy over an extended period of time and those same friendly, intelligent, wonderful people... will become as nasty and as violent as the most bloodthirsty Klingon. You don't believe me? Look at those faces. Look in their eyes.

I can live with it.

Lwaxana: Life's true gift is the capacity to enjoy enjoyment

TNG of course, S04E11, Data's Day

Riker: "Some days you get the bear, and some days the bear gets you.*"

I remember it with 'sometimes' instread of 'some days' and somehow it deeply resonates with me.

Capt. Picard:

The first duty of every Starfleet officer is to the truth, whether it's scientific truth or historical truth or personal truth! It is the guiding principle on which Starfleet is based. And if you can't find it within yourself to stand up and tell the truth about what happened, you don't deserve to wear that uniform!

—TNG, The First Duty

Mudd: "Human beings do not survive on bread alone ... but on the nourishments of liberty. For what indeed is a man without freedom ... naught but a mechanism, trapped in the cogwheels of eternity."

So many great lines to choose from. I’m watching Our Man Bashir right now and these stand out:

Garak: Kiss the girl, get the key. They never taught me that in the Obsidian Order.

A little later in the episode…

Bashir shoots at Garak grazing his neck.

Garak: Doctor, that was awfully close! What if you’d killed me?

Bashir: What makes you think I wasn’t trying?

Garak: Doctor, I do believe there’s hope for you yet.

Bashir: Ah, I’m so relieved. Now we have to get to the control room. Are you coming or not?

Garak: Well, who am I to question Julian Bashir, secret agent? Lead on!

Every one of us has a thousand different kinds of little people inside of us, and some of them want to get out and be wild, and some want to be sad or happy or inventive, or even just go dancing! That's why we all have so many different urges at different times. And all those different little people inside of us: we must never be afraid to take them with us wherever we go. Who knows when we may need one of them to pop up and rescue us from ourselves! Variety, my dear Alex. The great secret is not the variety of life, it's the variety of us.

Freedom!? That is our sacred word!

It is glemming out there.

You're mocking me aren't you? - Worf when I need a deadpan response to someone razzing me.for some reason.